EquipmentT200 BiaCore Surface Plasmon Resonance (SPR) Processing Unit

SPR is an optical phenomenon which allows monitoring binding of any two unlabeled molecules in real time. The SPR signal is based on changes in the refractive index at the surface of a gold sensor chip as an analyte flows in a microfluidic channel and binds to a ligand immobilized on the sensor chip. Monitoring the changes in the SPR signal over time results in a plot of the binding response versus time called sensorgram. Fitting of the sensorgram data allows calculation of binding parameters, such as KD, Kon and Koff. On top of the kinetic constants, SPR measurements can be applied for: Determination of specificity Studying thermodynamics of interaction Investigating mechanism of action Epitope characterization or affinity screening